tev protease cleavable 6xhis tag  (Addgene inc)

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components *

doi: 10.1074/mcp.M115.053165

Figure Lengend Snippet: Rvb1 and Rvb2 both associate with the other R2TP subunits Pih1 and Tah1, and copurify with a functionally diverse group of other protein complexes. A, components of the R2TP complex copurify with Rvb1 and Rvb2. Either Rvb1-TAP or Rvb2-TAP associated proteins were isolated from whole cell extracts of the strains indicated by TAP purification, and identified using MudPIT mass spectrometry. Relative amounts of each protein are estimated using dBNSAF values, where the dNSAF value for each protein was divided by the dNSAF value of the bait protein. The average relative amounts of the four R2TP subunits Rvb1, Rvb2, Pih1, and Tah1 are indicated (average dBNSAF values calculated from 3 biological replicates). Error bars indicate standard deviation. B, Rvb1/Rvb2 associated proteins. Proteins enriched in the Rvb1 and Rvb2 purified wild-type samples described in A, but not in control purifications were identified by PLGEM analysis as previously described (22) (FDR <0.05, except for: the snoRNP subunit Nop56; the CCT complex components Cct2, Cct6, Hsp42, Ssa1 and Ssa2). The area proportional Venn diagram was generated using “Venn Diagram Plotter” (PNNL, http://omics.pnl.gov/). C, protein complexes captured by Rvb1/2. For each complex the number of complex components captured (left) and the total number of known subunits for each complex (right) is indicated. D and E, dBNSAF values of CCT and proteasome complex components that are present in both Rvb1 and Rvb2 purifications.

Article Snippet: Rvb1 contained TEV (tobacco etch virus) protease cleavable 6xHis tag, Rvb2 contained FLAG tag and Hsp90 contained TEV protease cleavable Halo tag (Promega, Madison, WI) at their respective N termini.

Techniques: Isolation, Purification, Mass Spectrometry, Standard Deviation, Control, Generated

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components *

doi: 10.1074/mcp.M115.053165

Figure Lengend Snippet: Quantitative Proteomic Analysis of Top Rvb1/2 Interacting Proteins. A, Thirty six of the top proteins interacting with Rvb1 sorted from highest to lowest abundance after contaminant extraction. The focus of the graph is on proteins from the Ino80 complex (I), Swr1 complex (S), the Proteasome (P), the R2TP complex (R), and proteins in both Ino80 and Swr1 (B). The first 33 proteins are the 33 most abundant proteins found in the Rvb1-TAP purification after contaminant extraction. The average and standard deviation is shown for three biological replicates. B, Thirty six of the top proteins interacting with Rvb2 sorted from highest to lowest abundance after contaminant extraction. The focus of the graph is on proteins from the Ino80 complex (I), Swr1 complex (S), the Proteasome (P), the R2TP complex (R), and proteins in both Ino80 and Swr1 (B). The first 34 proteins are the 34 most abundant proteins found in the Rvb2-TAP purification after contaminant extraction. The average and standard deviation is shown for three biological replicates. C, The Copies per Cell information (36) was available for 35 of the 36 proteins listed in A and B (Tah1 missing from CpC data, while Act1, Hsc82 and Rpt5 were excluded for scale purpose). The inset graph is an expansion of the Ino80 (I) and Swr1 (S) specific components demonstrating the higher abundance of Ino80 proteins versus Swr1 proteins.

Article Snippet: Rvb1 contained TEV (tobacco etch virus) protease cleavable 6xHis tag, Rvb2 contained FLAG tag and Hsp90 contained TEV protease cleavable Halo tag (Promega, Madison, WI) at their respective N termini.

Techniques: Extraction, Purification, Standard Deviation

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components *

doi: 10.1074/mcp.M115.053165

Figure Lengend Snippet: Rvb1 and Rvb2 interact directly with Hsp90. A, Hsp90 copurifies with Rvb1/2 complexes purified from S. cerevisiae. Average dBNSAF values for Hsp90 are from the 3 biological replicates; error bars are standard deviation. B, recombinant Hsp90 expressed in Sf21 cells interacts with recombinant Rvb1/2 in the absence of other yeast proteins. Sf21 cells coinfected with baculoviruses encoding His-TEV-Rvb1, FLAG-Rvb2 and Halo-TEV-Hsp90 were prepared as described in Experimental Procedures. Affinity purifications were performed as shown in the purification scheme and various fractions were analyzed by SDS-PAGE and proteins visualized by staining with Coomassie Blue R-250. In addition, Western blot (on fraction F) and mass spectrometry (fraction K) analyses were performed to test for the presence of Rvb1, Rvb2 and Hsp90. Please note because one gel had high background, we scanned the Coomassie stained gel as a black and white image using the Odyssey Infrared Imaging system (LI-COR, Lincoln, NE), which improves the resolution of the gel. C, interaction between purified recombinant Hsp90 mixed with either purified Rvb1 or Rvb2. Sf21 cells were infected with baculoviruses encoding affinity tagged Rvb1, Rvb2 or Hsp90 and these proteins were affinity purified individually. Samples containing ∼50 μg of each protein were mixed and incubated for 30 min at room temperature in the combinations indicated. Mixtures were subjected to either His (Ni-NTA) purification or FLAG purification, the indicated fractions analyzed by SDS-PAGE, and proteins visualized by staining with Coomassie Blue R-250. Elution fractions from all 3 purifications (fractions M, N and O) were subjected to mass spectrometry analysis to confirm the presence of proteins of interest. Abbreviations: FT = flow through; W = wash; E = elution. Color coded arrows represent proteins presented in the purification scheme.

Article Snippet: Rvb1 contained TEV (tobacco etch virus) protease cleavable 6xHis tag, Rvb2 contained FLAG tag and Hsp90 contained TEV protease cleavable Halo tag (Promega, Madison, WI) at their respective N termini.

Techniques: Purification, Standard Deviation, Recombinant, SDS Page, Staining, Western Blot, Mass Spectrometry, Imaging, Infection, Affinity Purification, Incubation

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components *

doi: 10.1074/mcp.M115.053165

Figure Lengend Snippet: R2TP complexes lacking subunits Pih1 or Tah1 have an increased association with components of the RNA polymerase II and snoRNP complexes. A, a subset of proteins associate with Rvb1 and Rvb2 complexes purified without Pih1 or Tah1. Proteins present in the indicated purifications (described in Fig. 1), but not in control purifications, were identified using PLGEM analysis. The Venn diagram was generated using “Venny” (Oliveros, J.C. (2007–2015) Venny. An interactive tool for comparing lists with Venn's diagrams. http://bioinfogp.cnb.csic.es/tools/venny/). B, increased association of a set of proteins with Rvb1/2 in the absence of Pih1 or Tah1. Table I, representative proteins from the common 80 proteins shown in A, which are enriched in purifications using PIH1Δ or TAH1Δ deletion strains, compared with purifications using the wild-type strains. Average distributed spectral counts (dS) from three biological replicates are shown. Table II, RNAPII and snoRNP subunits are enriched in Rvb1/2 -TAP purifications using strains lacking the PIH1 or TAH1 genes (RNAPII subunits- Rpb1 and Rpb2: snoRNP subunits- Nop1 and Nop56).

Article Snippet: Rvb1 contained TEV (tobacco etch virus) protease cleavable 6xHis tag, Rvb2 contained FLAG tag and Hsp90 contained TEV protease cleavable Halo tag (Promega, Madison, WI) at their respective N termini.

Techniques: Purification, Control, Generated

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components *

doi: 10.1074/mcp.M115.053165

Figure Lengend Snippet: Interaction between Rvb1/2 and RNApolymerase II complex. A, left panel, TAP-purified samples of the indicated strains were analyzed by Western blotting. Rvb1/2 proteins were visualized with rabbit anti-TAP polyclonal antibodies and IRDye®800CW anti-rabbit secondary antibodies (green). Rvb1 associated proteins purified from S. cerevisiae lacking Pih1 are enriched for the pol II subunit Rpb1. A, right panel, Whole cell extracts prepared from the indicated yeast strains were analyzed for the presence of RNAPII subunit Rpb1 by fractionating samples using SDS-PAGE and detecting proteins by Western blotting. Rpb1 was detected with mouse anti-Rpb1 monoclonal antibodies and IRDye® 680LT anti-mouse secondary antibodies (red); tubulin was detected with rabbit anti-tubulin monoclonal antibodies and IRDye®800CW anti-rabbit secondary antibodies (green). Li-Cor Odyssey software was used for both imaging and band quantitation. Band quantitation was repeated on Western blots for three biological replicates; average values are plotted. B, Rpb1 association with Rvb1 or Rvb2 depends on Pih1 and Tih1. Average dBNSAF values were calculated from the purifications used in Fig. 1. C, the absence of Pih1 or Tah1 does not significantly affect Rpb1 mRNA levels. RNA samples purified from whole cell extracts of the indicated strains were analyzed by either RNA-seq or qPCR as described in Experimental Procedures. D, interaction between RNA polymerase II purified from yeast and either recombinant Rvb1 or Rvb2 isolated from Sf21 insect cells. Representative Silver stained gel showing purified RNAPII complex that was used as input (for inputs of Rvb1 and Rvb2 refer to Fig. 2C left panel). Approximately 10 μg samples of Rvb1, Rvb2 or RNAPII complex were mixed in the combinations indicated, affinity purified, and analyzed by SDS-PAGE and Western blotting.

Article Snippet: Rvb1 contained TEV (tobacco etch virus) protease cleavable 6xHis tag, Rvb2 contained FLAG tag and Hsp90 contained TEV protease cleavable Halo tag (Promega, Madison, WI) at their respective N termini.

Techniques: Purification, Western Blot, SDS Page, Bioprocessing, Software, Imaging, Quantitation Assay, RNA Sequencing, Recombinant, Isolation, Staining, Affinity Purification

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components *

doi: 10.1074/mcp.M115.053165

Figure Lengend Snippet: Comparison of Gene Expression Changes between Strains. A, RVB1-TAP and RVB2-TAP strains were compared with RVB1-TAP and RVB2-TAP strains with PIH1 and TAH1 deleted. Differentially expressed genes were identified using the DESeq2 package in R (25). Genes were considered differentially expressed if they had a p value<0.01 and a log2 fold change>0.7. An additional filtering removed lowly expressed genes with an average FPKM<10. The top genes were clustered with the default hierarchical clustering method in R (Raivo Kolde (2015). pheatmap: Pretty Heatmaps. R package version 1.0.2. http://CRAN.R-project.org/package = pheatmap) with a Euclidean distance measure. The heatmap created with the package pheatmap displays the data with standard row scaling to highlight the differences of individual genes across samples. B, The overlap of the top genes from each comparison are shown in the Venn diagram created with the Vennerable package in R (Jonathan Swinton (2013)). Vennerable: Venn and Euler area-proportional diagrams. R package version 3.0/r82. http://R-Forge.R-project.org/projects/vennerable/).

Article Snippet: Rvb1 contained TEV (tobacco etch virus) protease cleavable 6xHis tag, Rvb2 contained FLAG tag and Hsp90 contained TEV protease cleavable Halo tag (Promega, Madison, WI) at their respective N termini.

Techniques: Comparison, Gene Expression